A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells
Liu Ying
Zhou Jin
Wang Linlin
Hu Xiao-Xiao
Liu Xiangjun
Liu Meirong
Cao Zehui
Shangguan Dihua
Tan Weihong
· 2016
摘要：
Mitophagy is a process in which cells remove dysfunctional mitochondria and recycle their constituents in a lysosome-dependent manner.To probe this process,two different fluorescent dyes specific for mitochondria and lysosomes,respectively,are often used in combination.However,current fluorescent dyes for lysosomes cannot distinguish mitochondria-containing autolysosomes from other lysosomes.Therefore,we herein report a cyanine dye,HQO,which can simultaneously probe mitochondria and autolysosomes in live cells by exhibiting different fluorescence properties.HQO selectively accumulates in mitochondria but then transforms to the protonated HQOH
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